Functionalized coatings by electrospinning for anti-oxidant food packaging

The development of advanced formulations used for food packaging applications, which behave as protection or preservation materials and improve consumers’ health offers a route to reduced food wastage. The present study deals with investigations on the possibility of obtaining functionalized coatings by electrospinning of poly(ɛ-caprolactone), a synthetic biodegradable polymer together with vitamin E (α-tocopherol), selected as plant-based phenolic antioxidant. In this approach electrospinning allows the production of high surface area materials and thus offering an increased antioxidant activity. The electrospun fibres of poly(ɛ-caprolactone)/vitamin E were obtained, studied and their antioxidant properties were evaluated by measuring the fibre reactivity with 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical. The potential for extending the shelf-life of food products by using this approach is discussed

ASSESSMENT OF PEDIATRIC CRANIOCEREBRAL TRAUMA USING THE GLASGOW SCALE

Universitatea de Stat de Medicină şi Farmacie „Nicolae Testemiţanu”, Chişinău, Republica MoldovaIntroducere. Leziunile traumatice ale creierului (TCC) reprezintă o problemă majoră de sănătate publică și principala cauză de deces/dizabilități la nivel mondial în rândul copiilor/adolescenților. 1 din 20 de prezentări la Departamentul de Urgență al Spitalelor Pediatrice au fost cauzate de TCC, mai frecvent decât arsurile, otrăvirile. Scopul lucrării. Am evaluat starea de conștiință copiilor cu TCC la toate nivelurile de îngrijire medicală, neavând rezultatele TCC pediatrice inadecvat înregistrate în Republica Moldova. Material și metode. Un studiu prospectiv a fost pilotat în rândul copiilor cu TCC în SCM de Pediatrie „Valentin Ignatenco” din Chișinău pe o perioadă de 6 luni (01.03–31.08.2021). A fost aplicat un chestionar și un instrument electronic de colectare a datelor din fișele medicale Red Cap și analizat prin Epi Info 7. Rezultate. Numărul total de pacienți - 167. Băieți - 109, (65,3%) și 58 fetițe (34,7%). Vârsta medie de 9,0±5,04 ani. Prespitalicesc în conștiință (GCS 15p) - 57 copii (34,1%), în obnubilare moderată (13-14) - 103 copii (61,7%), în obnubilare profundă (11-12) - 4 copii (2,4%), sopor (8-10) - 3 copii (1,8%). Starea majorității s-a înrăutățit în timpul transportării. În DU, în starea conștientă - 30 copii (18%), obnubilare moderată – 102 (61,1%), obnubilare profundă – 25 (15%), sopor - 2 copii (1,2%), Coma I (67p) - 5 copii (3%) iar în Coma II profundă - 2 copii (1,2%). În urma tratamentului: GCS 15p - 155 copii (92,8%), 13-14 - 3 copii (1,8%). În Coma III depășită (3p) - decedat 9 copii (5,4%). Concluzii. Rezultatele au evidențiat lacune în îngrijiri medicale pentru TCC, care ne impun să reevaluăm și să adoptăm noi politici și linii directoare pentru prevenirea și tratamentul pacienților cu TCC.Background. Traumatic brain injury (TBI) - a major public health problems and the leading cause of death/disability worldwide among children/adolescents. One of every 20 Emergency Department presentations at Pediatric Hospitals is caused by a TBI, more common than burns or poisonings. Objective of the study. We evaluated the state of consciousness among children with TBI at all levels of medical care, as not yet having adequately registered outcomes of pediatric TBI. Material and Methods. A prospective study was piloted among the children of Municipal Pediatric Hospital „V. Ignatenco”, Chisinau, in 6 months (01.03-31.08.2021). A questionnaire and Red Cap electronic data collection tool was applied for medical records’ data collecting and analyzed thought Epi Info 7. Results. The total number of pediatric TBI patients - 167. The majority – 109 boys, (65.3%) and 58 girls (34.7%). The average age – 9.0±5.04 years old. At the pre-hospital period, being conscious (GCS 15p) - 57 children (34,1%), moderate obnubilation (13-14p) - 103 (61,7%), profound obnubilation (11-12p) - 4 (2.4%), sopor state (8-10p) - 3 (1,8%). The state of the TBI children worsened during the transportation. Coming to ED: in the conscious state - 30 children (18%), moderate obnubilation - 102 (61.1%), profound obnubilation – 25 (15%), sopor state 2 (1.2%), Coma I (6-7p) - 5 (3%) and Coma II - 2 (1,2%.). During the treatment, their state has changed: with GCS 15p - 155 children (92.8%), 13-14p - 3 (1.8%). Coma III exceeded state (3p) - 9 have died (5.4%). Conclusion. The results have shown some gapes in the provision of medical care of TBI that impose us to reevaluate and adopt some new politics of health and guidelines of TBI patients’ preventions and treatment

Development and characterization of novel films based on sulfonamide-chitosan derivatives for potential wound dressing

The objective of this study was to develop new films based on chitosan functionalized with sulfonamide drugs (sulfametoxydiazine, sulfadiazine, sulfadimetho-xine, sulfamethoxazol, sulfamerazine, sulfizoxazol) in order to enhance the biological effects of chitosan. The morphology and physical properties of functionalized chitosan films as well the antioxidant effects of sulfonamide-chitosan derivatives were investigated. The chitosan-derivative films showed a rough surface and hydrophilic properties, which are very important features for their use as a wound dressing. The film based on chitosan-sulfisoxazol (CS-S6) showed the highest swelling ratio (197%) and the highest biodegradation rate (63.04%) in comparison to chitosan film for which the swelling ratio was 190% and biodegradation rate was only 10%. Referring to the antioxidant effects the most active was chitosan-sulfamerazine (CS-S5) which was 8.3 times more active than chitosan related to DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging ability. This compound showed also a good ferric reducing power and improved total antioxidant capacity

Evaluarea traumatismului cranio cerebral pediatric folosind scala Glasgow

Background. Traumatic brain injury (TBI) - a major public health problems and the leading cause of death/disability worldwide among children/adolescents. One of every 20 Emergency Department presentations at Pediatric Hospitals is caused by a TBI, more common than burns or poisonings. Objective of the study. We evaluated the state of consciousness among children with TBI at all levels of medical care, as not yet having adequately registered outcomes of pediatric TBI. Material and Methods. A prospective study was piloted among the children of Municipal Pediatric Hospital „V. Ignatenco”, Chisinau, in 6 months (01.03-31.08.2021). A questionnaire and Red Cap electronic data collection tool was applied for medical records’ data collecting and analyzed thought Epi Info 7. Results. The total number of pediatric TBI patients - 167. The majority – 109 boys, (65.3%) and 58 girls (34.7%). The average age – 9.0±5.04 years old. At the pre-hospital period, being conscious (GCS 15p) - 57 children (34,1%), moderate obnubilation (13-14p) - 103 (61,7%), profound obnubilation (11-12p) - 4 (2.4%), sopor state (8-10p) - 3 (1,8%). The state of the TBI children worsened during the transportation. Coming to ED: in the conscious state - 30 children (18%), moderate obnubilation - 102 (61.1%), profound obnubilation – 25 (15%), sopor state - 2 (1.2%), Coma I (6-7p) - 5 (3%) and Coma II - 2 (1,2%.). During the treatment, their state has changed: with GCS 15p - 155 children (92.8%), 13-14p - 3 (1.8%). Coma III exceeded state (3p) - 9 have died (5.4%). Conclusion. The results have shown some gapes in the provision of medical care of TBI that impose us to reevaluate and adopt some new politics of health and guidelines of TBI patients’ preventions and treatment.Introducere. Leziunile traumatice ale creierului (TCC) reprezintă o problemă majoră de sănătate publică și principala cauză de deces/dizabilități la nivel mondial în rândul copiilor/adolescenților. 1 din 20 de prezentări la Departamentul de Urgență al Spitalelor Pediatrice au fost cauzate de TCC, mai frecvent decât arsurile, otrăvirile. Scopul lucrării. Am evaluat starea de conștiință copiilor cu TCC la toate nivelurile de îngrijire medicală, neavând rezultatele TCC pediatrice inadecvat înregistrate în Republica Moldova. Material și metode. Un studiu prospectiv a fost pilotat în rândul copiilor cu TCC în SCM de Pediatrie „Valentin Ignatenco” din Chișinău pe o perioadă de 6 luni (01.03–31.08.2021). A fost aplicat un chestionar și un instrument electronic de colectare a datelor din fișele medicale Red Cap și analizat prin Epi Info 7. Rezultate. Numărul total de pacienți - 167. Băieți - 109, (65,3%) și 58 fetițe (34,7%). Vârsta medie de 9,0±5,04 ani. Prespitalicesc în conștiință (GCS 15p) - 57 copii (34,1%), în obnubilare moderată (13-14) - 103 copii (61,7%), în obnubilare profundă (11-12) - 4 copii (2,4%), sopor (8-10) - 3 copii (1,8%). Starea majorității s-a înrăutățit în timpul transportării. În DU, în starea conștientă - 30 copii (18%), obnubilare moderată – 102 (61,1%), obnubilare profundă – 25 (15%), sopor - 2 copii (1,2%), Coma I (6- 7p) - 5 copii (3%) iar în Coma II profundă - 2 copii (1,2%). În urma tratamentului: GCS 15p - 155 copii (92,8%), 13-14 - 3 copii (1,8%). În Coma III depășită (3p) - decedat 9 copii (5,4%). Concluzii. Rezultatele au evidențiat lacune în îngrijiri medicale pentru TCC, care ne impun să reevaluăm și să adoptăm noi politici și linii directoare pentru prevenirea și tratamentul pacienților cu TCC

New Hybrid Materials Synthesized with Different Dyes by Sol-Gel Method

One well-known method for hybrids synthesis with incorporated organic dyes is sol-gel method, which is based on the concept of molecular manipulation to design ceramics, glasses, and composites. The low-temperature process allows for the incorporation of guest organic molecules within the inorganic matrix, as well as for the synthesis of hybrid networks in which the organic and inorganic phases are interpenetrating. The aim of the work presented in this paper was the preparation of the gels with three different dyes, at different molar ratios by using the hydrolytic sol-gel process. The interaction of the dye and the oxide was examined by UV-vis spectroscopy and FT-IR. The thermal stability of the hybrid organic-inorganic xerogel formed here was studied by thermal analysis. The micrographs obtained by scanning electron microscopy (SEM) revealed the high density of the films. Such characteristics indicate the possible application of these films in solar cells

Synthesis and Pharmacological Research Regarding New Compounds with Quinazolin-4-One Structure

The quinazoline scaffold is found in the chemical structure of many marketed drugs used in CNS disorders as antidepressants, anxiolytics, or hypnotics. Also, the carbamate ester derivatives have different certain therapeutic actions, such as hypnotic or parasympathomimetic ones. We have obtained new 4(3H)-quinazolinones by bringing together in the same structure the quinazoline nucleus and carbamate ester group. The compounds named Q1–Q5 were characterized by measuring the melting points, by determining the infrared and NMR spectra, and by elemental analysis. The pharmacological tests evidenced that the compounds have a very low acute toxicity, lethal doses being >2000 mg/kg bw. The compounds had different actions observed in forced swimming test (FST), tail suspension test (TST), or elevated plus maze (EPM), probably influenced by the presence of different radicals on the nucleus. Thus, Q1 with a nitro group in structure manifested the highest antidepressant effect, showing a reduction of immobilization time in FST and TST. On the other hand, Q3 and Q5, with two groups methoxy, respective ethoxy, had a slight anxiolytic effect, highlighted by an increase of the time spent in open arms and a decrease of the time spent in closed arms of EPM

The aspects of management in the acute severe pancreatitis

Clinica Chirurgie SCM „Sfînta Treime”, Catedra nr. 2 Chirurgie USMF „N.Testemiţanu” Secţia reanimare-terapie intensiva SCM „Sfînta Treime”The aims of this study was to estimate the results of the complex medico-surgical treatment on severe acute pancreatitis in concordance of the elaborates principles. Our results demonstrate the efficiency of the proposed protocol, including medical and supportive care, the rationale combination of endoscopic and surgical intervention. Imagistic USG and radiologic (CT) monitoring provides to accurate selection on oportun operative time and diminished the mortallity rate in patinets with severe acute pancreatitis. Obiectivul studiului dat a constituit estimarea rezultatelor tratamentulului complex monitorizat medico-chirurgical al pancreatitelor acute severe conform principiilor elaborate. Rezultatele obţinute au demonstrat eficacitatea protocolului propus, ce a inclus tratament conservativ intensiv, combinat raţional cu intervenţii endoscopice şi chirurgicale. Monitoringul USG şi radiologic (TC) au contribuit cu acurateţe selecţiei timpului operator oportun şi micşorării ratei de mortalitate la bolnavii cu pancreatite acute severe

Periostin in ovarian carcinoma: from heterogeneity to prognostic value

Introduction. Periostin (POSTN), an extracellular matrix protein, is involved in tumor-associated extracellular matrix (ECM) remodeling. However, its potential value as a prognostic and/or predictive factor has not yet been confirmed. The present study aims to assess POSTN expression separately in tumor cells and stroma of different ovarian carcinoma (OC) histological types, and its relationship with clinicopathological features. Material and methods. 102 cases of different histological OC subtypes were immunohistochemically investigated, for POSTN expression assessment in both epithelial tumor cells and tumor stroma. Statistical analysis was performed to correlate POSTN profile with clinicopathological characteristics, therapeutic response, and survival. Results.  POSTN expression in epithelial tumor cells was significantly correlated with POSTN expression in tumor stroma. The expression of POSTN in tumor cells was associated with histological type, tumor type (type I and II), tumor recurrence, progression-free survival (PFS), and overall survival (OS), whereas stromal POSTN expression was significantly correlated with age, histological type, tumor type, grade, and stage, residual disease, tumor recurrence, response to chemotherapy, and OS. Survival analysis revealed significant differences of PFS and OS in patients with high POSTN expression in tumor cells and negative stromal POSTN expression compared to patients with low POSTN expression in tumor cells and positive stromal POSTN expression (PFS: hazard ratio (HR) = 2.11, 95% confidence interval (CI): 1.33–3.37, P = 0.002; OS: HR = 1.78, 95% CI: 1.09–2.89, P = 0.019). Conclusions. The comparative assessment of POSTN immunoexpression in two tumor compartments: in tumor cells and stroma, by use of different scoring systems revealed that higher stromal POSTN levels are evidently correlated with unfavorable clinical features and poorer prognosis, while POSTN expression in tumor cells seems to be associated with a better patient outcome